John Towneley was the son of Charles Towneley, second son of Sir John Towneley. Mary was the grand daughter of Richard Towneley, eldest son of Sir John Towneley. Sir Richard Towneley, her father, was John Towneley's cousin. For a detailed account of the events leading up to their wedding in 1557 see Tracing the Towneleys 2004.
An oil painting John and Mary Towneley and Family, 1601 is displayed in the Towneley Room.